侵袭转移相关因子在胃肠道间质瘤中的检测及其意义

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世界华人消化杂志 2010年3月18日; 18(8): 791-797
ISSN 1009-3079 CN 14-1260/R
临床研究 CLINICAL RESEARCH
侵袭转移相关因子在胃肠道间质瘤中的检测及其意义
赵 毅, 王 强, 邓 鑫, 赵 滢, 张天彪
赵毅, 王强, 邓鑫, 赵滢, 中国医科大学附属盛京医院胃肠外
科 辽宁省沈阳市 110004
张天彪, 中国医科大学分子生物学教研室 辽宁省沈阳市
110001
赵毅, 副教授, 副主任医师, 2004年沈阳中国医科大学博士, 主
要从事胃肠肿瘤及缺血损伤研究.
辽宁省教育厅基金资助项目, No. 2009A726
作者贡献分布: 此课题由王强与赵毅设计并指导实验完成; 研究
过程由张天彪、邓鑫及赵滢操作完成; 数据分析由赵毅与张天
彪完成; 本论文写作由赵毅、张天彪及王强完成.
通讯作者: 王强, 主任医师, 教授, 110004, 辽宁省沈阳市和平区
三好街36号, 中国医科大学附属盛京医院胃肠外科.
wangq@http://wendang.chazidian.com
收稿日期: 2009-12-14 修回日期: 2010-01-26
接受日期: 2010-02-01 在线出版日期: 2010-03-18
Signicance of expression of
metastasis-related factors
uPA, MMP-2, MMP-9 and TGF-β1
in gastrointestinal stromal
tumors
Yi Zhao, Qiang Wang, Xin Deng, Ying Zhao,
Tian-Biao Zhang
Yi Zhao, Qiang Wang, Xin Deng, Ying Zhao, Department
of Gastrointestinal Surgery, Shengjing Hospital of China
Medical University, Shenyang 110004, Liaoning Province,
China
Tian-Biao Zhang, Department of Molecular Biology, China
Medical University, Shenyang 110004, Liaoning Province,
China
Supported by: the Foundation of Education Department of
Liaoning Province, No. 2009A726
Correspondence to: Professor Qiang Wang, Department
of Gastrointestinal Surgery, Shengjing Hospital of China
Medical University, Shenyang 110004, Liaoning Province,
China. wangq@http://wendang.chazidian.com
Received: 2009-12-14 Revised: 2010-01-26
Accepted: 2010-02-01 Published online: 2010-03-18
Abstract
AI M: To i nvest i gat e t he si gni f i cance of
expression of urokinase-type plasminogen
activator (uPA), matrix metalloproteinase-2
(MMP-2), matrix metalloproteinase-9 (MMP-9)
and transforming growth factor-β1 (TGF-β1) in
gastrointestinal stromal tumors (GISTs).
METHODS: The expression of uPA, MMP-2,
MMP-9 and TGF-β1 mRNAs and proteins in 124
GISTs specimens was detected by reverse tran-
scription-polymerase chain reaction (RT-PCR)
and Western blot, respectively.
RESULTS: In gastrointestinal stromal tumors,
the mRNA expression of uPA, MMP-2, MMP-9
and TGF-β1 is closely correlated with tumor
stage (NIH classication), mucosal invasion and
lymphatic metastasis. The mRNA expression
levels of uPA, MMP-2, MMP-9 and TGF-β1 were
up-regulated with increasing tumor stage, show-
ing significant differences among low-, mod-
erate- and high-risk GISTs. The positive rates
of uPA, MMP-2, MMP-9 and TGF-β1 mRNA
expression was not correlated with age, sex and
histological type. Similar results were obtained
for the expression of uPA, MMP-2, MMP-9 and
TGF-β1 proteins in GISTs. The expression of
uPA protein is positively correlated with that
of TGF-β1, MMP-2 and MMP-9 (r = 0.356, 0.323
and 0.346, respectively).
CONCLUSI ON: The positive activation of
TGF-β1 in GISTs possibly up-regulates uPA
expression which in turn activates MMP-2 and
MMP-9. The activation of MMP-2 and MMP-9
may lead to the degradation of extracellular ma-
trix degradation and promote tumor invasion
and metastasis.
Key Words: Gastrointestinal stromal tumor; Urok-
inase-type plasminogen activator; Matrix metal-
loproteinase-2; Matrix metalloproteinase-9; Trans-
forming growth factor-β1; Invasion and metastasis
Zhao Y, Wang Q, Deng X, Zhao Y, Zhang TB. Signicance
of expression of metastasis-related factors uPA, MMP-2,
MMP-9 and TGF-β1 in gastrointestinal stromal tumors.
Shijie Huaren Xiaohua Zazhi 2010; 18(8): 791-797
摘要
目的: 研究uPA、MMP-2、MMP-9、TGF-β1
等侵袭转移相关因子在胃肠道间质瘤(GISTs)
中的表达及其临床病理意义.
方法: 应用RT-PCR及Wes t er n bl ot法检测
uPA、MMP-2、MMP-9、TGF-β1在124例
GISTs中的表达.
结果: 在GISTs中, 随着NIH分级增高, uPA、
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■背景资料
胃 肠 道 间 质 瘤
(GISTs)是一类独
立起源于胃肠道
原始间质干细胞
呈非定向分 化的
消化系间叶肿瘤,
占全部胃肠道恶
性肿瘤的2 . 2 %.
GISTs主要发生血
行转移, 极少经淋
巴转移, 且其治疗
措施单一, 手术治
疗是其主要的治
疗手段, 术后放化
疗均不敏感. 研究
GISTs的侵袭与转
移对肿瘤的治疗
具有重要意义.
■同行评议者
张国梁, 主任医
师, 天津市第一
中心医院消化内
科