细胞角蛋白19mRNA在口腔鳞状细胞癌中表达的研究

华西口腔医学杂志 第27 卷 第3 期 2009 年6 月
West China Journal of Stomatology Vol．27 No. Jun2009
·基础研究·
[ 收稿日期] 2008-11-20； [ 修回日期 ] 2009-02-2
[ 基金项目] 泸州市科技局科研课题基金资助项目（泸市科200402号）
[ 作者简介] 冯燕（1978-），女，四川人，主治医师，硕士
[ 通讯作者] 聂敏海，Tel：80-217
[文章编号] 1000－1182（2009）03－0241-0
细胞角蛋白19 mRNA在
口腔鳞状细胞癌中表达的研究
冯燕
1
古亚兰
2
聂敏海
1
张齐梅
1
梁尚争
2
（1泸州医学院附属口腔医院 口腔内科； 2口腔颌面外科， 四川 泸州 4000）
[摘要] 目的 探讨口腔鳞状细胞癌（OSCC）患者癌组织中细胞角蛋白（CK）19 mRNA的变化和其发生的可能机制
以及CK9 mRNA检测的临床应用价值。方法 收集未接受过放疗和化疗的2例OSCC患者的手术标本（包括癌组织
2块、癌旁组织2块和颈清扫的淋巴结4枚），采用荧光定量聚合酶链式反应（FQ-PCR）法检测组织内CK9 mRNA
的表达，比较CK9 mRNA在上述组织中的相对表达量。结果 CK9 mRNA在OSCC癌组织内表达比其在正常口腔
黏膜内表达高85倍，比其在癌旁组织内表达高倍。9例患者颈清扫淋巴结内CK9 mRNA表达阳性，阳性率为
45%（9/2），而9例患者的22枚淋巴结中CK19 mRNA表达阳性率是818%（18/22），占2例患者3枚淋巴结的19%
（18/3）。淋巴结CK19 mRNA阳性患者的癌组织与癌旁组织和正常口腔黏膜的表达量比CK19 mRNA阴性患者低。
结论 CK19 mRNA在OSCC癌组织中的表达高于其在癌旁组织和正常口腔黏膜内的表达，可能是由于癌组织中
CK19的合成明显增加所致。淋巴结中CK19 mRNA的表达可以作为检测OSCC淋巴结微转移的指标之一，运用FQ-
PCR法检测淋巴结中CK19 mRNA的表达来检测OSCC的淋巴结微转移比普通病理法检测更灵敏。
[关键词] 口腔鳞状细胞癌； 细胞角蛋白19； 荧光定量聚合酶链式反应； 微转移
[中图分类号] R7398 [文献标识码] A
Study on the expression of cytokeratin 9 mRNA in oral squamous cell carcinoma FENG Yan
1
, GU Ya-lan
2

NIE Min-hai
1
 ZHANG Qi-mei
1
 LING Shangzheng
2
. （1. Dept. of Oral Medicine, The ffiliated Dental Hospital
of Luzhou Medical College, Luzhou 4000 China; 2 Dept. of Oral and Maxillofacial Surgery, The ffiliated
Dental Hospital of Luzhou Medical College, Luzhou 646000 China）
[ Abstract] Objective To elucidate the possible mechanism of oral carcinogenesis and to explore the value of
clinical application of the detection of cytokeratin（CK）19 for oral squamous cell carcinoma（OSCC）patients Methods
The cancerous tissues para-cancerous tissues and excised lymph nodes were collected from 2 operated patients with
OSCC The patients didn′ t receive radiotherapy and chemotherapy before hospitalization The relative expression of
CK19 mRNA in those tissues was detected by fluorescent quantitative polymerase chain reaction（FQ-PCR） Results
The expression of CK19 mRNA in the cancerous tissues was 185 and 1 times higher than that in normal oral
mucosa and in para-cancerous tissues respectively The expression of CK19 mRNA in lymph nodes from 9 patients
with OSCC was positive and the positive rate was 45（9/2） The positive rate of CK19 mRNA in all lymph nodes
from 9 patients with OSCC was 818（18/22） and the positive rate of CK19 mRNA in all lymph nodes from 2
patients with OSCC was 419（18/4） CK19 mRNA level in the cancerous tissues relative to para-cancerous tissues
and normal oral mucosa of the patients whose CK19 mRNA expression was positive was lower than that of the
patients whose CK19 mRNA expression was negative in lymph nodes respectively Conclusion The possible reason
that the expression of CK19 mRNA in the cancerous tissues was higher than that in para -cancerous tissues and
normal oral mucosa was that the CK19 synthesis in cancerous tissues increased obviously The detection of CK19
mRNA in lymph nodes was regarded probably as one of the markers for detecting OSCC micrometastasis in lymph
nodes The detec tion of CK19 mRNA in lymph nodes by FQ-PCR was more sensitive than hematoxylin-eosin staining
in diagnosing OSCC micrometastasis
[Key words] oral squamous cell carcinoma； cyto-
keratin 19； fluorescent quantitative polymerase chain
reaction； micrometastasis
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