F离子传感
内容需要下载文档才能查看 内容需要下载文档才能查看
AnalyticaChimicaActa539(2005)
内容需要下载文档才能查看311–316
Acolorimetricmethodfor?uoridedeterminationinaqueoussamples
basedonthehydroxyldeprotectionreactionofacyaninedye
Chang-QingZhua,Jin-LongChenb,HongZhenga,Yu-QinWub,Jin-GouXua,?
a
TheKeyLaboratoryofAnalyticalScienceofMOE,DepartmentofChemistry,XiamenUniversity,Xiamen361005,China
bCollegeofChemistryandMaterialsScience,AnhuiNormalUnversity,Wuhu241000,China
Received6January2005;receivedinrevisedform3March2005
Availableonline30March2005
Abstract
Anewhighlysensitiveandselectivecolorimetricmethodfor?uoridedeterminationinwaterisdescribed.Thenovelreagentforthismethodisacyaninedye(C1)onwhichthehydroxylgrouphasbeenprotectedbyreactionwithtert-butyldimethylsilane(TBS)toformthesilanateddye,C2.C2isselectivelyattackedby?uorideionstoreformC1.C1hasanabsorptionmaximumat600nmwithamolarabsorptivityofabout200,000.Underoptimizedconditions,absorbanceat600nmisproportionalto?uorideconcentrationuptoabout1×10?4mol/Lwithadetectionlimitof1.0×10?7mol/L.Becauseofthespeci?caf?nityof?uoridefortheTBSfunctionalgrouponC2,thereislittleinterferencebyotherions.Themethodhasbeensuccessfullyappliedfor?uoridedeterminationsinlocalrainwatersamples.Resultsdeterminedbytheproposedmethodagreefavorablywiththosedeterminedbya?uorideionselectiveelectrodemethod.©2005ElsevierB.V.Allrightsreserved.
Keywords:Fluorideion;Cyanine;Hydroxyldeprotectionreaction;Colorimetricmethod
1.Introduction
Theeffectof?uorideonbothhumansandanimalshasadualroleasanessentialelementandathighlevelsasatoxicsubstance[1].Excess?uorideionresultsin?uorosisandrenal,gastrointestinalandimmunologicaltoxicity[2].Also,plantsandfruitssuchasapricots,plumsgrapes,tulips,irisandsweetcornaresensitiveto?uoride[3].Hydrogen?uorideisoftenfoundinrainwater[4]asaresultofvariousindustrialprocesses,suchasphosphatefertilizerproduction,aluminiumproductionandmetalsmelting.Therefore,anac-curateandrapidmethodforthedeterminationof?uorideinrainwatersamplesisimportantforassessmentofairandwaterquality.
Severalanalyticaltechniqueshavebeendevelopedanddescribedfor?uoridedeterminationsinwaterincludingspec-trophotometry[5–7],?uorometry[3,8–10],potentiometry
?
Correspondingauthor.Tel.:+865922180307;fax:+865922188054.E-mailaddress:jgxu@http://wendang.chazidian.com(J.-G.Xu).
(ion-selectiveelectrodes(ISE)[11–13],ionchromatography(IC)[14],gaschromatography(GC)[15],capillaryzoneelectrophoresis(CZE)[16]andradioanalysis[17].Amongthese,ISEmethodsaremostwidelyappliedbecausetheyareeasytouse,selectiveandprovideawidedynamicrange[11].Determinationoflow-concentration?uoridebyISEcanbedif?cultduetolongequilibrationtimes,electrodedriftanddissolutionofthelanthanum?uoridemembranecrystal[18,http://wendang.chazidian.comparedtoISE?uoridedeterminationmethods,chromatographicmethodsaretimeconsuming[15]andrequiremoreexpensiveinstrumentation.Severalspectrometricmethods[20–22]arewidelyacceptedforthedeterminationoftrace?uorideinnaturalwatersduetotheirsimplicity,lowcostandreliability.Inmostofthesespectro-metricmethods,?uorideionsdisplaceorganicligandsfromcoloredcomplexesofmetalionssuchaszirconium,thorium,titanium,aluminiumandferriciron[20–22].Displacementofthemetalionororganicligandby?uoridethereforecausestheabsorbanceofthereactionmixturetodecrease.Often,however,Beer’slawisnotobeyedinsuchmethods
0003-2670/$–seefrontmatter©2005ElsevierB.V.Allrightsreserved.doi:10.1016/j.aca.2005.03.002
312C.-Q.Zhuetal./AnalyticaChimicaActa539(2005)311–316
duetothediversityofco-existingcomplexesof?uoridewiththemetalioninthesolution.Furthermore,manyanionsorcationscompetewith?uorideorthecentralmetalionforcoordination[7],whichresultsinseriousinterference.
Recently,Descalzoetal.[23],KimandSwager[24]re-portedanewreactionsystemfortheselectivedetectionof?uorideionthatexploitstheuniquechemicalreactivityof?uorideionswithsilicon.Longreactiontimesandorganicsolventrequirementsofthesereactionsystems[24]greatlylimitedtheirutilityforwateranalysisapplications.Inthispaper,wedescribeusinganewreagentfor?uoridedetermi-nationinaqueoussolution,basedontheselectiveattackof?uorideontheSi–Obondofthecyaninedye,C2(1-ethyl-4-(p-tert-butyldimethylsilaneetherstyryl)quinoliniumiodide).C2isconvenientlyobtainedin98%yieldbyreactionofcya-ninedye,C1(1-ethyl-4-(p-hydroxystyryl)quinoliniumio-dide)withtert-butyldimethylsilane(TBS)asshowninFig.1.ThelargemolarabsorptivityofC1(≈200,000Lcmmol?1)[25]makesitanexcellentchromophoricreporter.WhenC2isincubatedwith?uorideionsin7:3v/vTHF:watersolutions,thecharacteristicabsorptionbandofC1at600nmgraduallyappears.Underoptimizedconditions,alinearrelationshipbe-tweentheabsorbanceat600nmand?uorideionconcentra-tionisobserved.Thisanalyticalapproachprovidesahighlysensitiveandselectivecolorimetricmethodforthedetermi-nationof?uorideioninaqueoussolutionthatwasappliedtothedetectionof?uorideioninlocalrainwatersamples.In-terferencebyforeignanionsisnegligibleandresultsbytheproposedmethodcomparefavorablywithresultsbystandardF?ISEmethods.
2.2.Reagents
The?uoridestandardsolutionswerepreparedbyase-rialdilutionofa10?2mol/Lsodium?uoridestocksolu-tion.CyaninedyeC1wassynthesizedandpuri?edaccord-ingtoBlazsek-Bodoetal.[26,27]andsilanatedwithTBSasdescribedbySartorietal.[28](Fig.1),toformtheindicator,C2in98%yield.C2wasdissolvedinchloro-formsolutiontomakea1.7×10?3mol/Lstocksolution.ThepurityofC2wascon?rmedby1HNMRandHRMSspectra.Allchemicalswereanalyticalreagentgradeandusedasreceived.Doublydeionizedwaterwasusedforallexperiments.
2.3.Pretreatmentofsamples
Twolocalrainwatersampleswereobtainedafterapre-treatmentaccordingtotheliterature[29];twotapwatersam-plesweredilutedby10-foldwithdoublydeionizedwaterforcomparativeexperiment.
2.4.Standardprocedureforthedetectionof?uorideAddsequentiallyto5.0mLvolumetric?askscontaining3.5mLofTHFsolution,0.1mLofC2workingsolution,1.3mLofstandardsodium?uoridesolutionorsampleand0.1mLofNaH2PO4–NaOHbuffersolution(pH9.0).Mixthecontentsofthe?askthoroughly.After10min,measuretheabsorbanceat600nmusingTHF–water(7:3,v/v)solutionasthereference.Afterdeterminationsarecomplete,recycleTHFbysimplecollectionanddistillationtoreducethepos-siblepollution.
2.Experimental2.1.Apparatus
AHitachiU-3010spectrophotometer(Tokyo,Japan)wasusedforrecordingabsorptionspectraandmakingabsorptionmeasurements.ThepHwasmeasuredwithaModelpHs-3cmeter(Shanghai,China).A201?uorideIonSelectiveElec-trode(JiangshuElectroanalysisApparatusFactory,China)wasusedforthedetectionof?uorideinrainwatersamples.
3.Resultsanddiscussion3.1.Spectralcharacteristics
ThevisibleabsorptionspectraofC1inpH9.0bufferdis-playstwobandsasshowninFig.2b.AtlowpH,however,thespectraofC1displayonlyonevisiblebandat440nmasshowninFig.3thatcorrsespondstotheabsorptionof
内容需要下载文档才能查看the
Fig.1.Protectionanddeprotectionofcyanine’shydroxyl.
C.-Q.Zhuetal./AnalyticaChimicaActa539(2005)311–316
内容需要下载文档才能查看313
Fig.2.AbsorptionchangeofC2uponadditionofNaF:(a)aserialvisualcolorchange,the?uorideconcentrations(fromthelefttotheright)are:0.0,2.8×10?6,6.6×10?6,9.4×10?6,2.8×10?5,4.7×10?5,6.6×10?5and9.4×10?5mol/L.(b)UV–visabsorptionspectrainTHF–water(70:30,v/v)solvent.Concentrationsof?uorideare:0.0,4.8×10?6,9.4×10?6,2.8×10?5,4.8×10?5,6.6×10?5and9.4×10?5mol/L.Otherconditionsarethesameasthosedescribedinthe
内容需要下载文档才能查看procedure.
Fig.3.UV–visabsorptionchangeofthecyaninewithpHbefore(C1)andafter(C2)itshydroxywasprotected.Solvent:THF–water(70:30,v/v);con-centrationofC1andC2:3.4×10?5mol/L;NaH2PO4–NaOHbuffer:pH6.0–9.7.
specieswithnon-ionizedhydroxylgroup.Thebandat440nmwasassignedtoanintramolecularchargetransfertransition[30].ItsintensitydecreasesaspHincreasesabove7.Thein-tensityofthebandat600nmincreasesasthepHincreasesabovepH7.Thebehaviorcanbeexplainedasfollows.WiththeincreaseofpHthehydroxylprotonbeginstoionize,whichincreasestheproportionoftheionizedforminsolutionduetomakingoftheenergybarrieroftheintramolecularCTsmaller.
Theabsorptionbandat600nmofC2atpH9.0disap-peared,withalittleblueshiftingofthemainabsorptionbandat440nm(Fig.2b)duetotheincreaseoftheenergybar-rieroftheintramolecularCTafterthehydroxylgroupofC1wasprotected.Atthesametime,thedependencetopHdis-appearedsinceonlyonecyanineformexisted.AsshowninFig.3,C2didnotshowtheabsorptionatabout600nmevenatthepHupto9.7.
WhenC2wasincubatedwithvariousamountsof?uorideion,adramaticcolorchangefromyellowtogreenwasob-served(Fig.2a)andthecharacteristicabsorptionofinitialcyanineC1atabout600nmgraduallyreturnedtothesolu-tion(Fig.2b).Undertheoptimumconditions,therewasagoodlinearrelationshipbetweentheabsorbanceat600nmandtheconcentrationof?uorideion.3.2.EffectsofreactionmediumandpH
THFgreatlyincreasestherateof?uoridereactionwithC2.Experimentsshowedthat70%THFwasoptimalforspeci?creactionof?uoridewithC2.BecauseC2wassta-bleandsolubleinchloroformatroomtemperature,chlo-roformwasusedtopreparethestocksolutionofC2andthissmallamountofchloroform(<3%)isunavoid-ablyintroducedintothedetectionsystem.Experimentsshowedthatitseffectonanalyticalresultswasnegligi-ble.
Twobuffersystems,Tris–HClandNaH2PO4–NaOHbuffer,wereinvestigated.Thelattershowedaverylowblankabsorbanceat600nm(seeFig.2b).Therefore,0.1mol/LpH9.0NaH2PO4–NaOHbuffersolutionwaschoseninordertohavehighersensitivityandlowerbackground.3.3.ProbeC2concentrationandincubationtime
TheeffectofconcentrationofC2onthedeterminationof?uoridewasinvestigated.Whenconcentrationsofotherreagentswerekeptconstant,theabsorbancedifferencebe-tweentheabsenceandthepresenceof?uorideionin-creasedwithincreasingtheamountofC2.However,whentheconcentrationofC2exceeded3.4×10?5mol/L,thebackgroundabsorbance(without?uorideion)increasedac-cordingly.Thus,C2concentrationof3.4×10?5mol/Lwasrecommended.
Undertheseoptimizedconditions,completecleavageoftheSi–Obond(ortheassociationof?uoridewiththesiliconatom)onC2requiredabout1.5h(Fig.4).Althoughanalyti-
314C.-Q.Zhuetal./AnalyticaChimicaActa539(2005)
内容需要下载文档才能查看311–316
Fig.4.Effectofreactiontime.Concentrationsof?uoride(fromthebottomtothetop):0.0,4.8×10?6and4.8×10?5mol/L.Otherconditionsarethesameasthosedescribedintheprocedure.
calsensitivitycouldhavebeenincreasedwithlongerreactiontimes,anincubationtimeof10minwaschosenforthisworkasapracticalmatter.Ourexperimentsdemonstratethataccu-rateresultscouldbeobtainedatthisrelativelyshortreactiontime.
3.4.Interferenceofforeignions
Theeffectsofforeignionsonthedeterminationof1.6×10?6mol/L?uorideindeionizedwaterwerestudied.Foreignionconcentrationsthatresultinrelativeerrorslessthan±5%arelistedinTable1.FromTable1,itcanbeseenthatthetestedionshaveverylittleinterferencewith?thedeter-mination.2?TherelativelyhighconcentrationsofCl,Br?,I?,SO4,SCN?andPO43?thatcanbetoleratedareexplained
Table1
ToleranceofforeignsubstancesForeignionsConcentrationRelativeerror(10?6mol/L)caused(%)Cl?280?5.0Br?125?1.7I?
78?1.0SO42?80+2.5SCN?90+3.5PO43?200+1.0Fe3+50?3.0NH4+200?2.0Cr3+50+3.5Co2+70+2.5Ni2+45+3.5Ca2+80?1.5Ba2+100+2.8Cu2+100?3.5Al3+40?2.0Mn2+
60
+4.0
Concentrationof?uoride:1.6×10?6mol/L.Otherconditionsarethesameasthosedescribedinthe
内容需要下载文档才能查看procedure.
Fig.5.Calibrationgraphsfordifferentincubationtime(10minand30min).Absorbancewasmeasuredat600nm;solvent:THF–water(7:3,v/v).Otherconditionsarethesameasthosedescribedintheprocedure.
bythespeci?citywithwhich?uoridereactswiththesiliconatomonC2.
3.5.Calibrationgraphsandanalysisofsamples
Thecalibrationgraphsfortwokindsofincubationtime,i.e.10minand30min,wereconstructed(Fig.5)undertheoptimumconditions.FromFigs.4and5,itcanbeseenthatreasonablyprolongingthereactiontimecanimprovethean-alyticalsensitivity.
Thelimitof7detection(LOD)fortheproposedmethodwas1.0×10?mol/Lgivenbytheequation,LOD=KS0/S,whereKisanumericalfactorchosenaccordingtothecon-?denceleveldesired,S0thestandarddeviationoftheblankmeasurements(n=6)andSthesensitivityofthecalibrationgraph.Hereavalueof3forKwasused.Andtherelativestandarddeviation(n=6)was3.3%forthedeterminationof1.6×10?6mol/LNaF.
Theproposedmethodwasappliedtothedeterminationof?uorideinrainwatersamples.Twolocalrainwatersampleswerecollectedand?lteredaccordingtotheliterature[29].ThedeterminationresultsbythismethodandcomparisonwiththatobtainedbytheF?ISEstandardmethodareshowninTable2.AllthedeterminationresultsshowninTable2werethemeanofsixmeasurements.Therecoverytestwascarriedoutbyadding1.0×10?6mol/L?uoridestandardsolutioninthesamplesolutions.Therecoverieswerefoundtobe98–102%.Atthesametime,thedatashowninTable2,whichareveryclosetothatobtainedbytheF?ISEstandardmethod,arealsoinagreementwiththatprovidedbylocalenvironmentmonitorstation.Furthermore,twotapwatersamplesdilutedby10-foldwithdoublydeionizedwaterweredetectedbytheproposedmethodandF?ISEmethod,theresultsareshowninTable3.FromthedataofTables2and3,itcanbeseenthattheproposedmethodissensitiveandreliable.
C.-Q.Zhuetal./AnalyticaChimicaActa539(2005)311–316
Table2
AnalyticalresultsofrainwatersamplesRainwatersamplesno.12
ThismethodF?,founda(n=6)3.78±0.103.55±0.11
R.S.D.(%)2.53.0
F?,added(10?6mol/L)1.01.0
F?,found(10?6mol/L)4.804.53
Recovery(%)10298
F?ISEmethodF?,found(10?6mol/L)3.853.50
315
t-testb1.811.15
Theanalyticalresultisexpressedasx¯±(tp,n?1S/),wheretp,n?1=t0.95,5=2.57,x¯andSarethemeanandstandarddeviation,respectively.Theunitfortheconcentrationof?uorideinrainwaterisexpressedin10?6mol/L.bt
0.95,5=2.57;t0.95,5=2.02.
a
Table3
ComparativedeterminationofF?intapwatersamplesSample
ThismethodF?,founda(10?6mol/L)F?founda
Tapwater1
1.21
Tapwater2
1.10
ab
F?ISEmethodF?,found(10?6mol/L)
Recovery(%)1049998101
F?foundaUndetectedUndetected
F?added1.04.01.04.0
F?foundbUndetected4.25Undetected4.16
Recovery(%)–106–104
F?added1.04.01.04.0
F?foundb2.255.202.085.15
The?uorideintapwaterwith10-folddilution.Meanofsixdeterminations.
Thetotal?uoridein10-folddilutedtapwaterwiththeadditionof?uoride.Meanofsixdeterminations.
4.Conclusions
Anovelcolorimetricmethodforthedeterminationof?uorideionhasbeendevelopedbasedontheselectiveat-tackof?uorideonasilanatedcyaninedye.Themethodhasseveralmeritsthatinclude:(i)highsensitivityandspeci?city;(ii)semi-quantitativedeterminationsbyopti-calcomparisonispossible;and(iii)synthesisofC1andC2arestraightforwardandspectrophotometricoropticalcomparisondetectionmethodsareconvenientandeconomi-cal.
Acknowledgements
ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina(NNSFC,No.29775021)andtheEd-ucationCommissionNaturalScienceFoundationofAnhuiProvince(No.2001KJ114ZD);alltheauthorswishtoex-presstheirgratitude.
References
[1]TheImpactofFluorideonHealthJ.Am.Diet.Assoc.100(2000)
1208–1213;
TheImpactofFluorideonHealthJ.Am.Diet.Assoc.101(2001)126–131.
[2]D.Purves,TraceElementContaminationoftheEnvironment,Else-vier,Amsterdam,1977,pp.79–82(Chapter3).
[3]D.H.Chen,M.D.LuquedeCastro,M.Valcarcel,Anal.Chim.Acta
230(1990)137.
[4]A.G.T.Marc,HoopVanden,F.M.J.Rob,J.J.Cleven,StadenVan,
J.Neele,J.Chromatogr.A739(1996)241.
[5]StandardMethodsfortheExaminationofWaterandWastewater,
Method4500FD,20thed.,AmericanPublicHealthAssociation,Washington,1998,pp.4–62.
[6]EPAMethodsforChemicalAnalysisofWaterandWastes,Method
340.1,USEnvironmentProtectionAgency,Washington,1978.[7]E.Bellack,P.Schouboe,Anal.Chem.30(1958)2032.[8]M.Garrido,A.G.Lista,M.Palomeque,B.S.Fern´andezBand,Talanta
58(2002)849.
[9]T.L.Har,T.S.West,Anal.Chem.43(1971)136.
[10]J.Nishimoto,T.Yamada,M.Tabata,Anal.Chim.Acta428(2001)
201.
[11]M.S.Frant,J.W.RossJr.,Science154(1966)1553.
[12]D1179–99StandardTestMethodsforFluorideIoninWater,ASTM
BookofStandards,vol.11.01,ASTMInternational,WestCon-shohocken,PA,USA,2003.
[13]H.Hara,Chun-chingHuang,Anal.Chim.Acta338(1997)141.[14]L.N.Moskvin,A.N.Katruzov,T.G.J.Nikitina,Anal.Chem.53
(1998)173.
[15]M.Yuwono,S.Ebel,Arch.Pharm.Pharm.Med.Chem.330(1997)
348–352.
[16]R.Bodor,V.Madajova,D.Kaniansky,M.Masar,M.Johnck,B.J.
Stanislawski,J.Chromatogr.A916(2001)155.
[17]D.I.Paik,D.S.Ma,D.Y.Park,H.S.Moon,Y.I.Chang,J.B.Kim,J.
Radioanal.Chem.217(1997)221–223.
[18]R.C.Hawkings,L.P.V.Corriveau,S.A.Kushneriuk,P.Y.Wong,Anal.
Chim.Acta102(1978)61.
[19]C.Fuchs,D.Dom,C.A.Fuchs,H.V.Henning,C.Mcintosh,F.
Scheler,Clin.Chim.Acta60(1975)157–167.
[20]M.A.Faraj-Zadeh,E.G.Kalhor,Microchim.Acta137(2001)
169.
[21]Z.J.Huang,Y.L.Yang,G.Y.Yang,J.Y.Yin,FenxiHuaxue27(1999)
1363.
[22]I.Nemcova,B.Evtimova,J.Hrachovska,Dokl.Bolg.Akad.Nauk.
43(1990)45.
[23]A.B.Descalzo,D.Jim´enez,J.E.Haskouri,D.Beltr´an,P.Amor´os,
?ez,J.Soto,http://wendang.chazidian.commun.(2002)M.D.Marcos,R.Mart´?nez-M´an
562.
[24]T.-H.Kim,T.M.Swager,Angew.Chem.Int.Ed.42(2003)4803.[25]G.Patonay,M.D.Antonie,Anal.Chem.63(1991)321.
下载文档
热门试卷
- 2016年四川省内江市中考化学试卷
- 广西钦州市高新区2017届高三11月月考政治试卷
- 浙江省湖州市2016-2017学年高一上学期期中考试政治试卷
- 浙江省湖州市2016-2017学年高二上学期期中考试政治试卷
- 辽宁省铁岭市协作体2017届高三上学期第三次联考政治试卷
- 广西钦州市钦州港区2016-2017学年高二11月月考政治试卷
- 广西钦州市钦州港区2017届高三11月月考政治试卷
- 广西钦州市钦州港区2016-2017学年高一11月月考政治试卷
- 广西钦州市高新区2016-2017学年高二11月月考政治试卷
- 广西钦州市高新区2016-2017学年高一11月月考政治试卷
- 山东省滨州市三校2017届第一学期阶段测试初三英语试题
- 四川省成都七中2017届高三一诊模拟考试文科综合试卷
- 2017届普通高等学校招生全国统一考试模拟试题(附答案)
- 重庆市永川中学高2017级上期12月月考语文试题
- 江西宜春三中2017届高三第一学期第二次月考文科综合试题
- 内蒙古赤峰二中2017届高三上学期第三次月考英语试题
- 2017年六年级(上)数学期末考试卷
- 2017人教版小学英语三年级上期末笔试题
- 江苏省常州西藏民族中学2016-2017学年九年级思想品德第一学期第二次阶段测试试卷
- 重庆市九龙坡区七校2016-2017学年上期八年级素质测查(二)语文学科试题卷
- 江苏省无锡市钱桥中学2016年12月八年级语文阶段性测试卷
- 江苏省无锡市钱桥中学2016-2017学年七年级英语12月阶段检测试卷
- 山东省邹城市第八中学2016-2017学年八年级12月物理第4章试题(无答案)
- 【人教版】河北省2015-2016学年度九年级上期末语文试题卷(附答案)
- 四川省简阳市阳安中学2016年12月高二月考英语试卷
- 四川省成都龙泉中学高三上学期2016年12月月考试题文科综合能力测试
- 安徽省滁州中学2016—2017学年度第一学期12月月考高三英语试卷
- 山东省武城县第二中学2016.12高一年级上学期第二次月考历史试题(必修一第四、五单元)
- 福建省四地六校联考2016-2017学年上学期第三次月考高三化学试卷
- 甘肃省武威第二十三中学2016—2017学年度八年级第一学期12月月考生物试卷
网友关注
- [教学]体育教研员若何适应新一轮基础教导课程改革
- 长沙四大名校初一分班考试试卷一
- 生命科学教学研究如何凸显"研究"
- 初中区县学情概况41
- 阿基米德原理
- 农村中学数学有效课堂浅议
- 第7章 体育教学方法论
- 【精品】基础写作96
- 高二英语必修5(外研版)6-2reading
- 语文版七年级下册教案全集
- 哲学与人生教案6599749682
- 高职汽车类专业教学改革的探究
- 人教版八年级上册物理教案
- 格律诗词写作基础及其实践
- 体育教学论总复习
- 八年级数学教案
- 【北师大版】七年级上册数学教案全套【七年级上教案全套】(可编辑)
- 第三章G带染色体识别
- 人教版高一必修1数学教案:精品全套
- 七年级下册中图版地理教案
- 华东师大版九年级数学上册教材分析
- 文言文阅读-虚词
- 高中历史必修三知识点总结 人教版371710426
- 2.2奇妙的二氧化碳(第2课时)助学案
- 第1课时建立一元二次方程模型导学案
- 什么是美术作品
- 苏教版教学课件高二生物精品课件:215免疫(苏教版必修3)(共46张PPT)
- 初中数学基础知识及经典题型完整版(实用的中考专题复习指导书)
- 《健康评估》教学大纲
- 宁夏石嘴市惠农中学七年级数学上册 3.1《多姿多彩的图形》课件(人教新课标版)
网友关注视频
- 外研版英语三起6年级下册(14版)Module3 Unit2
- 冀教版英语三年级下册第二课
- 沪教版牛津小学英语(深圳用) 四年级下册 Unit 7
- 第12章 圆锥曲线_12.7 抛物线的标准方程_第一课时(特等奖)(沪教版高二下册)_T274713
- 北师大版八年级物理下册 第六章 常见的光学仪器(二)探究凸透镜成像的规律
- 3月2日小学二年级数学下册(数一数)
- 飞翔英语—冀教版(三起)英语三年级下册Lesson 2 Cats and Dogs
- 【部编】人教版语文七年级下册《老山界》优质课教学视频+PPT课件+教案,安徽省
- 七年级英语下册 上海牛津版 Unit3
- 沪教版八年级下册数学练习册一次函数复习题B组(P11)
- 沪教版牛津小学英语(深圳用) 五年级下册 Unit 10
- 冀教版小学数学二年级下册第二单元《租船问题》
- 沪教版牛津小学英语(深圳用) 六年级下册 Unit 7
- 【部编】人教版语文七年级下册《逢入京使》优质课教学视频+PPT课件+教案,安徽省
- 北师大版数学四年级下册第三单元第四节街心广场
- 苏科版数学八年级下册9.2《中心对称和中心对称图形》
- 青岛版教材五年级下册第四单元(走进军营——方向与位置)用数对确定位置(一等奖)
- 19 爱护鸟类_第一课时(二等奖)(桂美版二年级下册)_T3763925
- 三年级英语单词记忆下册(沪教版)第一二单元复习
- 七年级英语下册 上海牛津版 Unit9
- 沪教版牛津小学英语(深圳用) 四年级下册 Unit 8
- 苏科版八年级数学下册7.2《统计图的选用》
- 小学英语单词
- 30.3 由不共线三点的坐标确定二次函数_第一课时(市一等奖)(冀教版九年级下册)_T144342
- 沪教版牛津小学英语(深圳用)五年级下册 Unit 1
- 冀教版小学数学二年级下册1
- 19 爱护鸟类_第一课时(二等奖)(桂美版二年级下册)_T502436
- 沪教版八年级下册数学练习册21.3(2)分式方程P15
- 【部编】人教版语文七年级下册《泊秦淮》优质课教学视频+PPT课件+教案,湖北省
- 二年级下册数学第二课
精品推荐
- 2016-2017学年高一语文人教版必修一+模块学业水平检测试题(含答案)
- 广西钦州市高新区2017届高三11月月考政治试卷
- 浙江省湖州市2016-2017学年高一上学期期中考试政治试卷
- 浙江省湖州市2016-2017学年高二上学期期中考试政治试卷
- 辽宁省铁岭市协作体2017届高三上学期第三次联考政治试卷
- 广西钦州市钦州港区2016-2017学年高二11月月考政治试卷
- 广西钦州市钦州港区2017届高三11月月考政治试卷
- 广西钦州市钦州港区2016-2017学年高一11月月考政治试卷
- 广西钦州市高新区2016-2017学年高二11月月考政治试卷
- 广西钦州市高新区2016-2017学年高一11月月考政治试卷
分类导航
- 互联网
- 电脑基础知识
- 计算机软件及应用
- 计算机硬件及网络
- 计算机应用/办公自动化
- .NET
- 数据结构与算法
- Java
- SEO
- C/C++资料
- linux/Unix相关
- 手机开发
- UML理论/建模
- 并行计算/云计算
- 嵌入式开发
- windows相关
- 软件工程
- 管理信息系统
- 开发文档
- 图形图像
- 网络与通信
- 网络信息安全
- 电子支付
- Labview
- matlab
- 网络资源
- Python
- Delphi/Perl
- 评测
- Flash/Flex
- CSS/Script
- 计算机原理
- PHP资料
- 数据挖掘与模式识别
- Web服务
- 数据库
- Visual Basic
- 电子商务
- 服务器
- 搜索引擎优化
- 存储
- 架构
- 行业软件
- 人工智能
- 计算机辅助设计
- 多媒体
- 软件测试
- 计算机硬件与维护
- 网站策划/UE
- 网页设计/UI
- 网吧管理