提高脊神经根损伤后神经元存活率：我们能做什么？

热休克蛋白27在细胞应激反应中可起到保护神经元的重要作用。课题组以往研究表明,在脊神经根拔除后的1周,热休克蛋白27阳性大运动神经元(>500 μm2)存活率增加。更进一步的实验发现,在脊神经根轻微压碎后热休克蛋白27在运动神经元中的表达增强。中国南京医科大学李林博士所在课题组进行的一项研究首创的采用了椎管外拔除腰神经根,并造成大量前角神经元变性死亡的实验动物模型

SICENCE新闻发布 作者：李林 稿号：N201303020 提高脊神经根损伤后神经元存活率：我们能做什么？

热休克蛋白27在细胞应激反应中可起到保护神经元的重要作用。课题组以往研究表明，在脊神经根拔除后的1周，热休克蛋白27阳性大运动神经元(>500 μm2)存活率增加。更进一步的实验发现，在脊神经根轻微压碎后热休克蛋白27在运动神经元中的表达增强。中国南京医科大学李林博士所在课题组进行的一项研究首创的采用了椎管外拔除腰神经根，并造成大量前角神经元变性死亡的实验动物模型，对比单纯拔除和先压挫后拔除两种方法对运动神经元存活及热休克蛋白27的表达的影响，证实预先压挫所导致的神经保护机制可能是通过增加热休克蛋白27的上调和减弱一氧化氮合酶上调而实现的。文章发表在《中国神经再生研究（英文版）》杂志2014年3月第5期。

免疫荧光染色显示，预先压挫配合大鼠脊髓L4节段脊神经根拔除可使大鼠腹侧运动神经元中热休克蛋白27 (箭头)表达增加

Article: " Preconditioning crush increases the survival rate of motor neurons after spinal root avulsion," by Lin Li, Yizhi Zuo, Jianwen He (Department of Human Anatomy, Nanjing Medical University, Nanjing, Jiangsu Province, China) Li L, Zuo YZ, He JW. Preconditioning crush increases the survival rate of motor neurons after spinal root avulsion. Neural Regen Res. 2014;9(5):540-548. 欲获更多资讯：

How to increase the survival rate of motor neurons after spinal root avulsion

A previous study showed that, 1 week after avulsion of the spinal nerve root, small motor neurons (< 500 μm2) negative for 27 kDa heat shock protein (HSP27) immunoreactivity died and only large (> 500 μm2) HSP27-positive motor neurons survived in the spinal cord ventral horn. This was followed by the enhancement of HSP27 expression in motor neurons observed after mild crush of the spinal nerve root. Dr. Lin Li and co-workers from Nanjing Medical University in China investigated whether preconditioning crush can increase the survival rate of motor neurons, which is the first to

demonstrate that loss of ventral motor neurons after nerve root avulsion is partially prevented by preconditioning crush. This injury tolerance might act through the induction of HSP27 by preconditioning crush, which inhibits the upregulation of neuronal nitric oxide synthase to attenuate the cytotoxic effects of nitric oxide following crush. The relevant article has been published in the Neural Regeneration Research (Vol. 9, No. 5, 2014).

热休克蛋白27在细胞应激反应中可起到保护神经元的重要作用。课题组以往研究表明,在脊神经根拔除后的1周,热休克蛋白27阳性大运动神经元(>500 μm2)存活率增加。更进一步的实验发现,在脊神经根轻微压碎后热休克蛋白27在运动神经元中的表达增强。中国南京医科大学李林博士所在课题组进行的一项研究首创的采用了椎管外拔除腰神经根,并造成大量前角神经元变性死亡的实验动物模型

SICENCE新闻发布 作者：李林 稿号：N201303020

Five weeks after L4 nerve root avulsion with preconditioning crush 1 week prior to avulsion, the expression of 27 kDa heat shock protein is stronger than that in the avulsion-only group.

Article: " Preconditioning crush increases the survival rate of motor neurons after spinal root avulsion," by Lin Li, Yizhi Zuo, Jianwen He (Department of Human Anatomy, Nanjing Medical University, Nanjing, Jiangsu Province, China)

Li L, Zuo YZ, He JW. Preconditioning crush increases the survival rate of motor neurons after spinal root avulsion. Neural Regen Res. 2014;9(5):540-548.